An in vitro, light responsive, retinal model for accurate predictions of in vivo outcomes you can have confidence in.
Freshly isolated, in vitro, proximal tubule cell model, shipped directly to your lab for your kidney transporter studies.
aProximate™ is one of the more advanced, near physiological, in vitro, proximal tubule cell (PTC) models. Now available for shipment to customers to perform kidney transporter studies in-house.
Advantages of aProximate™
- Primary cell-derived model
- Derived from fresh human kidney samples
- Polarised PTC layer that forms tight junctions
- Retains expression of relevant kidney transporters
Scientific relevance of aProximate™ for transporter assays
aProximate™ expresses most relevant kidney transporters unlike other primary and immortalised PTCs such as RPTEC, KH2 and HEPTEC, which express very low or negligible levels. See table 1 below.
Table 1: Comparison of transporter expression levels in aProximate™ and RPTECs
Product description and usage
aProximate™ Assay-ready plates (Human) are provided in a 24-well Transwell® format. The cells are shipped in stasis media and require revival at 37oC prior to use. Cell recovery is assessed by measuring TEER (transepithelial electrical resistance). When TEER measurements reach a minimum of 60 Ω.cm2, the cells are ready to assay. Recovery of cells usually takes between 1 and 3 days. The cells must be revived immediately upon receipt and must not be frozen.
Product Specification
| Format | 24 well Transwell® plates |
| Cell Type | Primary proximal tubule epithelial cells |
| Species Origin | Human |
| Characteristics | Polarised Monolayer |
| Assay Window | Recommended within 3 days of plate receipt |
| Quality Control | Tested negative for: HIV, HBcAb, HCV, SARS/CoV-2 |
| Shipping Temperature | 4oC. |
| Ordering Lead Time | 4-6 weeks |
The model is provided with maintenance medium and user guide with a detailed protocol for recovery of cells upon receipt. A TEER meter (not provided) is required to perform the measurements.
Revival of aProximate™ Assay-Ready Plates
Suggested applications:
- Kidney transporter assays
- Bidirectional transepithelial compound flux studies
- Compound uptake studies
- Target engagement and efficacy studies
Validation of aProximate™ tight junction formation and transporter expression:
Functional validation of aProximate™ for transporter assays:
aProximate™ Assay-Ready Plates (Human) are produced from human healthy primary kidney tissue which has been sourced from ethically approved locations. Further details on the ethical standards and procedures can be supplied on request.
