Lung Epithelia Model - Newcells

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The most advanced near-physiological high throughput kidney proximal tubule cells (PTC) model to investigate drug transport modalities in vitro.

Lung epithelia model

Lung model

A model to investigate airway physiology, viral infection, drug safety and environmental impacts on lung airway epithelia.

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Liver model

We are developing a model of liver sinusoid derived from human induced pluripotent stem cells (iPSC).

Lung Epithelia Model

A lung epithelia model to investigate airway physiology, viral infection, drug safety and environmental impacts on lung airway epithelia.

  • A characterised model containing all upper airway epithelium cells organised in a stratified epithelium
  • Expresses ACE2 proteins on the cell surface allowing replication of SARS-CoV-2
  • Responds to known airborne particles with cytokine responses and changes in gene expression

The airway epithelium is comprised of heterogenous, polarised epithelial cells which provide a first-line of defence against inhaled toxins, particles and viruses. Distinct airway epithelial cell populations are largely recaptured in Newcells induced pluripotent stem cell (iPSC)-derived respiratory airway epithelial model, whilst maintaining epithelial cell functionality including differentiation, fluid and mucin secretion and beating cilia.

Recapitulation of the complex airway epithelium using our iPSC-derived lung epithelia model allows for investigation of airway physiology and functionality, assessment of drug safety and the environmental impact on the airways. Additionally, our iPSC-derived airway model can be utilised for respiratory viral infection modelling.

iPSCs utilised in our models allow for an unlimited supply of airway epithelial cells, reducing potential for genetic and environmental impact variability often associated with primary human epithelial models.

Product specifications

Format· 3D heterogenous stratified epithelium culture on a permeable support (Thincert™/Transwell™)
Relevant Cell Types· Basal cells
· Goblet cells
· Club cells
· Ciliated epithelial cells
Species· Human

Possible applications for the lung epithelia model

  • Investigation of respiratory viral infections

As demonstrated during the Covid-19 pandemic, understanding viral biology and pathogenicity is crucial for the development of anti-viral therapies. A popular alternative to complex and low-throughput animal models, airway epithelial in vitro models allow the comprehensive study of lung based virology.

The SARS-CoV-2 virus binds to and is internalised via the angiotensin converting enzyme 2 (ACE2) expressed in Newcells 3D in vitro lung model, making it an effective model for studying SARS-CoV-2 infection and replication.

  • Assessment of respiratory drug safety and specialised, functional drug efficacy

Due to the large surface area of the lungs, they are highly susceptible to toxicity. In vitro models with limited cell types and functionality are not an effective way to model and measure drug safety. Safety studies exposing animals to high-dose concentrations and tracking toxic responses often don’t effectively replicate reactions in humans.

Newcells iPSC-derived lung epithelia model can be used to minimise off-target drug effects with novel approaches to safety screening.

Case study poster: https://newcellsbiotech.co.uk/wp-content/uploads/2021/04/Ivo-et-al-Optimisation-of-induced-pluripotent-stem-cell….-1.pdf

  • Toxicology testing of environment pollutants and other xenobiotic compounds

“Air pollution is the world’s most significant environmental health risk”

World Health Organisation

Current assessments of the impacts of air pollutants on human health are based on epidemiological studies, animal models and immortalised cell lines. However, since cell lines and animal models can poorly represent human physiology, and epidemiological studies alone are rarely able to prove the effects of air pollutants of human health, human in vitro lung models are vital in understanding environmental effects of inhaled toxins.

Newcells lung 3D in vitro model has been tested for inhalation toxicology with cigarette smoke condensate, cerium nanoparticles, busulfan and other environmental xenobiotics.

State-of-the-art 2D and 3D cell-based assays

Newcells can perform a variety of non-functional and functional analyses, including:

  • Gene and protein expression
  • Immunohistochemistry / immunocytochemistry
  • Cytokine/protein release and secretion
  • Epithelial barrier integrity and it’s response to damage
  • Mucin secretion
  • Airway surface liquid height
  • Ciliary beat frequency

RESOURCES:

Development of a physiologically relevant lung model for understanding SARS-CoV-2 infection https://www.drugtargetreview.com/article/77157/development-of-a-physiologically-relevant-lung-model-for-understanding-sars-cov-2-infection/

A human iPSC-derived model of SARS-CoV-2 infection and pathogenesis in the lung. Webinar.

Lung Model aids sars-cOv-2 research https://www.technologynetworks.com/cell-science/blog/lung-model-aids-sars-cov-2-research-333502

Optimisation of iPSC-derived lung airway models for toxicity testing and risk assessment evaluation https://newcellsbiotech.co.uk/wp-content/uploads/2021/04/Ivo-et-al-Optimisation-of-induced-pluripotent-stem-cell….-1.pdf

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