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Assay-Ready Plates aProximate™

aProximate™ Assay-Ready Plates

Advancing Drug Research with In Vitro Proximal Tubule Models Delivered To Your Lab

1.

Worldwide Shipping Ready to Go

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Versatile Applications including drug transporter studies

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Enhanced Efficiency with High Throughput

aProximate™ Proximal Tubule Assay-Ready Plates: Advancing Drug Research with Accurate In Vitro Models

aProximate™ Proximal Tubule Assay-Ready Plates offer an innovative approach to drug research. providing an accurate in vitro model of the proximal tubule derived from fresh human kidney tissue. Unlike other primary cell models and cell lines, these unique assay-ready plates are freshly isolated from human donor kidney tissue, never frozen or passaged, ensuring unparalleled accuracy and reliability in your experiments. With worldwide shipping options, our assay-ready plates are accessible to researchers across the globe and delivered to you ready for your assays.

“From a practical standpoint, the cell model of the aProximateTM assay ready plate was easy to handle. The monolayer showed a very good TEER, already one day after the revival.”  Alexandre Durrwell, Senior Scientist I, Novartis

Features and benefits

  • Accurately recapitulates the function of the proximal tubule
  • Global accessibility
  • Increased efficiency with high throughput
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A microscope image of a nephron model
Tight junctions between aProximate™ proximal tubule cells shown by ZO-1 staining

24 and 96 wells format

Freshly isolated PTCs

Validated model

“From a practical standpoint, the cell model of the aProximateTM assay ready plate was easy to handle. The monolayer showed a very good TEER, already one day after the revival.” Alexandre Durrwell, Senior Scientist I, Novartis

Formats

  • 24-well Transwells
  • 96-well Transwells

Cell types

  • aProximate™ - primary kidney proximal tubule cells

Species

  • Human

Revival of aProximate™ Assay Ready Plates

Why use Newcells aProximate™ kidney proximal tubule cell model? Close Open
  1. Reliable and Accurate Results: With the use of  primary human proximal tubule cells, our assay-ready plates offer trustworthy results, aiding in the assessment of drug transport, metabolism, and toxicity. This reliability supports the identification of promising drug candidates and minimises potential risks.
  2. A Realistic In Vitro Model: Emulate human physiology more realistically with our assay-ready plates, offering insights that align more closely with clinical outcomes and mechanistic understanding of renal drug handling.  The aProximate™ model facilitates more precise predictions of drug behaviour and effects due to higher expression of renal transporters compared to other models.
  3. Versatile Applications: aProximate™ Assay-Ready Plates support various applications, including drug transporter studies, ADME/DMPK evaluations, and toxicity and safety screenings. This versatility streamlines experimental processes and optimises resource utilisation.
  4. Expert Assistance: Our team of experts is committed to your success. We offer technical support and guidance to ensure that you obtain meaningful results with our assay-ready plates.
Receiving Your aProximate™ Assay-Ready Plates Close Open

Designed with your convenience in mind, our aProximate™ Assay-Ready Plates are ready to go and enable you to focus on your research without the hassle of extensive cell culture procedures.

We ship the primary proximal tubule cells in stasis media, a gel that preserves the cells’ viability during transit. These cells are maintained at a stable 4oC to ensure their optimal condition upon arrival. Our streamlined delivery process ensures the cells’ integrity, and we take pride in providing high-quality products that meet your research needs.

Once the Assay-Ready Plates are in your hands, a simple and user-friendly protocol guides you through the process of removing the stasis gel and replacing it with the supplied maintenance media. This step-by-step guide ensures a smooth transition of recovery for the proximal tubule cells, allowing you to establish a functional and polarized cell monolayer with ease. All proximal tubule cells included in our assay-ready plates undergo rigorous quality control testing for your peace of mind.

TEER measurements indicate when the cells have formed a confluent monolayer, signalling their readiness for experimentation. Thus a TEER (Trans-epithelial Electrical Resistance) meter is required to determine the optimal time for assay. Our user guide provides comprehensive details on using the TEER meter effectively to assess the monolayer’s confluency.

aProximate™ is available for shipment to customers to perform kidney transporter and toxicity studies in-house. aProximate™ Assay-Ready plates are provided in either 24-well or 96-well Transwell® format.

Format of aProximate™ Assay-Ready Plates Close Open
  • Human aProximate™ proximal tubule cells
  • Format : 24- or 96-well Transwell®
  • Shipped in stasis media (gel)
  • Fully validated shippment at 4oC
  • Require immediate attention and revival immediately upon receipt
The front cover of the aProximate ebook

eBook available

Read the renal proximal tubule cells platform eBook for more insights

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Images

A microscope image of a nephron model
ZO-1 Immunocytochemistry staining of PTCs (red) and Hoescht nuclear staining.
A graph showing different levels of creatine uptake
Functional validation of aProximate™ assay-ready plates for transporter assays Functional validation studies for transcellular flux and uptake of creatinine revealed preferential basolateral-to-apical (JBA) creatinine transport, indicative of normal physiological function. A JBA uptake ratio >1.5 indicates that the cells are functional.

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Is this the model for you? Speak to one of our experts about your requirements.

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