Newcells is releasing a new highly engineered MPS flow system fully compatible with all our in vitro cell-based models.
Newcells has launched a new engineered micro-physiological system (MPS) flow technology compatible with our in vitro cell-based models.
The technology was developed using fluid dynamics software and 3-D printing technology to enable physiological flow to be applied to kidney proximal cells in 96-well format. This elegant system uses a unique design of a plate holder precision engineered to work with Transwells®. The design means that cell layers can be subjected to flow without the need for specialised infrastructure and can be quickly adapted to any cell type that has been optimised for growth on Transwells®.
Studying in vitro complex models under flow without the need of specialised equipment
The MPS flow plates have been developed to fit 6, 24 and standard 96-well Transwell® inserts. The design creates two distinct compartments with one compartment subjecting a polarised cell model to flow. Any analytical readouts developed for well plates can be translated to work on this flow system. One key advantage is that the system allows a large quantity of cells to be subjected flow with benefits to sensitivity and range of assay outputs. The cells are seeded on the basal side of each Transwell®, and are subject to flow and shear forces between 0.1-2 dynes/cm2, generated by slow and constant movement obtained by placing the plates on a rocking device; without the need for complex equipment, pumps, and tubing.
Innovation by design
Using computational fluid dynamics modelling, we have determined the optimal shape and dimensions of the flow channel to obtain constant flow and maximal uniform shear stress exposure across the cell layers. We have researched different materials to select a non-absorptive PDMS-free material to minimise binding of non-specific small molecules, improving the sensitivity and quality of the data produced from the system. The MPS Flow plate is designed to enable us to process a large number of data points rapidly when used with 96-well Transwell® filters
It is now easier than ever to access MPS Flow technology without the need to invest in specialised equipment.
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