Resources

KIDNEY - RENAL TRANSPORT AND DRUG-DRUG INTERACTION

Papers

Review of the pharmacokinetics and metabolism of triclopyr herbicide in mammals: Impact on safety assessments

Michael Bartels, Colin Brown, Git Chung, Melissa Chan, Claire Terry, Sean Gehen, Marco Corvaro
Regulatory toxicology and pharmacology. 2020; 116:104714

Keywords

INTER-SPECIES DIFFERENCES | TRICLOPYR | TOXICOKINETICS

The importance of incorporating OCT2 plasma membrane expression and membrane potential in IVIVE of metformin renal secretory clearance

Vineet Kumar, Jia Yin, Sarah Billington, Bhagwat Prasad, Colin D A Brown, Joanne Wang, Jashvant D Unadkat
Drug Metabolism and Disposition. 2018; 46(10):1441-1445

Keywords

QUANTITATIVE PROTEOMICS | IN VITRO-TO-IN VIVO EXTRAPOLATION | METFORMIN

The limitations of renal epithelial cell line HK-2 as a model of drug transporter expression and function in the proximal tubule

Sarah E. Jenkinson, Git W Chung, Ellen van Loon, Nur S Bakar, Abigail M Dalzell, Colin D A Brown
Pflügers Archiv European Journal of Physiology. 2012; 464(6): 601-11

Keywords

HK-2 CELLS | RENAL CLEARANCE | qPCR

The breast cancer resistance protein transporter ABCG2 is expressed in the human kidney proximal tubule apical membrane

M. Huls, C D A Brown, A S Windass, R Sayer, J J M W van den Heuvel, S Heemskerk, F G M Russel, R Masereeuw
Kindey International. 2008; 73(2): 220-5

Keywords

BREAST CANCER RESISTANCE PROTEIN | ABCG2 | RENAL DRUG EXCRETION

Abundance of drug transporters in the human kidney cortex as quantified by quantitative targeted proteomics

Bhagwat Prasad, Katherine Johnson, Sarah Billington, Caroline Lee, Git W Chung, Colin D A Brown, Edward J Kelly, Jonathan Himmelfarb, Jashvant D Unadkat
Drug Metabolism and Disposition. 2018; 46(3): 279-290

Keywords

UPTAKE TRANSPORTERS | EFFLUX TRANSPORTERS | EFFLUX TRANSPORTERS

In vitro models for accurate prediction of renal tubular xenobiotic transport in vivo

Jelle Vriend, Keith R. Pye, Colin Brown.
Current Opinion in Toxicology. 2021; 25: 15-22

Keywords

DRUG TRANSPORT | DRUG-DRUG INTERACTIONS | IN VITROIN VIVO EXTRAPOLATION

Derivation of a system-independent Ki for p-glycoprotein mediated digoxin transport from system-dependent IC50 data

Aqsaa Chaudhry, Git Chung, Adam Lynn, Akshata Yalvigi, Colin Brown, Harma Ellens, Michael O’Connor, Caroline Lee, Joe Bentz
Drug Metabolism and Disposition. 2018; 46(3): 279-290

Keywords

IC50 | P-GLYCOPROTEIN | IN VIVO DDI PREDICTIONS

Characterisation of human tubular cell monolayers as a model of proximal tubular xenobiotic handling

Colin D. A. Brown, Rachel Sayer, Amy S Windass, Iain S Haslam, Marc E De Broe, Patrick C D’Haese, Anja Verhulst
Toxicology and Applied Pharmacology. 2008; 233(3): 428-438

Keywords

RENAL XENOBIOTIC SECRETION | PROXIMAL TUBULE | ABC TRANSPORTERS

Articles

Improved in vitro renal tubular drug transport modelling to meet regulatory and development cost challenges

Dr Colin Brown
European Pharmaceutical Review. December 2020

Keywords

DRUG DEVELOPMENT | RENAL PROXIMAL TUBULE EPITHELIA | DRUG TRANSPORT

Posters and poster presentations

Primary human proximal tubule cell monolayers as a predictive model of renal phosphate homeostasis in heath and disease

Colin D. A. Brown.
Drug Metabolism and Pharmacokinetics. 2017; 32(1): S14-S15

Keywords

PHOSPHATE HANDLING | TENOFOVIR | HUMAN PROXIMAL TUBULE CELL (hPTC) MONOLAYERS

Complementary in vitro tools to investigate renal drug transport

Viktoria Juhasz, Erzsébet Beéry, Zsuzsanna Gaborik, Gábor Imre, Colin D A Brown, Joseph Zolnerciks and Berend Oosterhuis
Drug Metabolism and Pharmacokinetics. 2017; 32(1): S71

Keywords

PRIMARY PTC MONOLAYER ASSAY | RENAL ELIMINATION | DOUBLE TRANSFECTED

Improved phenotype of human proximal tubule epithelial cells in a 3D microfluidic model

Jelle Vriend, Keith Pye, Git Chung, Mike Nicholds, Lyle Armstrong, Colin Brown
SOT 2020

Keywords

CHARACTERIZATION | POLARISATION | PTECs

Technical sheet

aProximate™

Technical sheet

KIDNEY - RENAL DRUG SAFETY

Papers

c-Rel orchestrates energy-dependent epithelial and macrophage reprogramming in fibrosis

Jack Leslie, Marina García Macia, Saimir Luli, Julie C. Worrell, William J. Reilly, Hannah L. Paish, Amber Knox, Ben S. Barksby, Lucy M. Gee, Marco Y. W. Zaki, Amy L. Collins, Rachel A. Burgoyne, Rainie Cameron, Charlotte Bragg, Xin Xu, Git W. Chung, Colin D. A. Brown, Andrew D. Blanchard, Carmel B. Nanthakumar, Morten Karsdal, Stuart M. Robinson, Derek M. Manas, Gourab Sen, Jeremy French, Steven A. White, Sandra Murphy, Matthias Trost, Johannes L. Zakrzewski, Ulf Klein, Robert F. Schwabe, Ingmar Mederacke, Colin Nixon, Tom Bird, Laure-Anne Teuwen, Luc Schoonjans, Peter Carmeliet, Jelena Mann, Andrew J. Fisher, Neil S. Sheerin, Lee A. Borthwick, Derek A. Mann, Fiona Oakley
Nature Metabolism. 2020; 2:1350–1367

Keywords

FIBROSIS | C-REL | Pfkfb3

Development of a novel predictive in vitro non human primate proximal tubule model for drug transporter and nephrotoxicity studies

Colin Brown
Drug Metabolism and Pharmacokinetics. 2019; 34(1):S53

Keywords

CROSSSPECIES COMPARISONS | NON HUMAN PRIMATE | PROXIMAL TUBULE

aProximateTM as a novel, predictive model of aminoglycoside-induced nephrotoxicity

Keith Pye, Git Chung, Lyle Armstrong, Mike Nicholds, Colin Brown
Abstracts of the 55th Congress of the European Societies of Toxicology (EUROTOX 2019) TOXICOLOGY SCIENCE PROVIDING SOLUTIONS. Toxicol Lett. 2019;314:S167–8.

Keywords

AMINOGLYCOSIDES | CLINICALLY RELEVANT BIOMARKERS | TRANS-EPITHELIAL ELECTRICAL RESISTANCE

Freshly isolated primary human proximal tubule cells as an in vitro model for the detection of renal tubular toxicity

Piyush Bajaj, Git Chung, Keith Pye, Tomoya Yukawa, Akio Imanishi, Yuichi Takai, Colin Brown, Matthew P.Wagoner
Toxicology. 2020; 442:152535

Keywords

CLUSTERIN | DRUG INDUCED KIDNEY INJURY (DIKI) | NEPHROTOXICITY

Articles

In vitro platforms for de-risking nephrotoxicity during drug development

Dr Sandy Primrose & Dr Colin Brown
Drug Target Review. March 2020

Keywords

KIDNEY TRANSPORTER FUNCTION | DRUG DEVELOPMENT | DRUG-DRUG INTERACTIONS

Webinars

Reducing compound attrition by predicting renal tubular toxicity with in vitro PTEC models

Dr Piyush Bajaj & Dr Colin Brown
September 2020

Keywords

DIKI | COMPOUND ATTRITION | TUBULAR TOXICITY

Predictive in vitro primary proximal tubule models for understanding nephrotoxicity in drug development programs

Dr Colin Brown
February 2020

Keywords

DRUG DEVELOPMENT | SPECIES DIFFERENCES | NEPHROTOXICITY

Posters and poster presentations

P52 – Determination of aminoglycoside toxicity in human renal proximal tubule monolayers using clinical relevant biomarkers of nephrotoxicity

Colin Brown, Lyle Armstrong, Mike Nicholds, Git Nicholds
Drug Metabolism and Pharmacokinetics. 2020; 35(1):S37

Keywords

RENAL TOXICITY | AMINOGLYCOSIDE | TRANSEPITHELIAL RESISTANCE

Characterization of cisplatin toxicity in aProximate™ human proximal tubule cell monolayers

Colin Brown, Git Chung, Lyle Armstrong, Mike Nicholds
Drug Metabolism and Pharmacokinetics. 2019; 34(1): S52-S53; SOT 2019 P105

Keywords

CISPLATIN CHALLENGE | GENE EXPRESSION | TRANSEPITHELIAL ELECTRICAL RESISTANCE

Human primary proximal tubule cell monolayers as a novel predictive model of nephrotoxicity

Git Chung, Sarah Billington, R. D. Wilde, Colin Brown
Toxicology Letters. 2016;258:S139-S140

Keywords

hPTCs | TRANS-EPITHELIAL ELECTRICAL RESISTANCE | TOXICOLOGY SCREENING

Development of a predictive in vitro human proximal tubule platform for de-risking nephrotoxicity

Piyush Bajaj, Git Chung, Keith Pye, Mike Nicholds, Yvonne Dragan, Matthew Wagoner, Colin Brown

Keywords

NEPHROTOXIN CHALLENGE | HUMAN PTC MONOLAYERS | NEPHROTOXICITY

Development of predictive in vitro cross-species proximal tubule models for nephrotoxicity studies

Git Chung, Colin Brown, Lyle Armstrong, Mike Nicholds

Keywords

NEPHROTOXIN CHALLENGE | HUMAN PTC MONOLAYERS | NEPHROTOXICITY

An inter-species comparison of the triclopyr in vitro and in vivo toxicokinetic properties, for risk assessment purpose

Marco Corvaro, Michael Bartels, Colin Brown, Git Chung, Melissa Chan
SOT 2019

Keywords

INTER-SPECIES DIFFERENCES | TRICLOPYR | NEPHROTOXICITY

The aProximate™ in vitro renal proximal tubule cell model as a platform to investigate the renal uptake and nephrotoxic liability of biologics

Colin D.A. Brown, Keith Pye, Mike Nicholds, Git Chung

Keywords

MEGALIN | CUBULIN | BIOLOGICS

Technical sheet

aProximate™

Technical sheet

RETINA

Papers

Differentiation of retinal organoids from human pluripotent stem cells

Valeria Chichagova, Birthe Dorgau, Majed Felemban, Maria Georgiou, Lyle Armstrong, Majlinda Lako
Current Protocols in Stem Cell Biology. 2019; 50(1):e95

Keywords

DIFFERENTIATION | iPSCs | RETINAL ORGANOIDS

Using statistical models to identify key variables critical for generation of retinal organoids from iPSC

Valeria Chichagova, Dean Hallam, Mike Nicholds, Robert Thomas, Majlinda Lako, Lyle Armstrong
Investigative Ophthalmology & Visual Science. 2018; 59(9): 565

Keywords

RETINAL ORGANOIDS | iPSCs | STATISTICAL MODELS

Human iPSC differentiation to retinal organoids in response to IGF1 and BMP4 activation is line- and method-dependent

Valeria Chichagova, Gerrit Hilgen, Ali Ghareeb, Maria Georgiou, Madeleine Carter, Evelyne Sernagor, Majlinda Lako, Lyle Armstrong
Stem Cells. 2020; 38(2):195-201

Keywords

RETINAL ORGANOIDS | DIFFERENTIATION | iPSCs

Enhancing immune function of hiPSC-derived retinal organoids by incorporating microglial cells

Valeria Chichagova, Maria Georgiou, Birthe Dorgau, Madeleine Carter, Git Chung, Evelyne Sernagor, Lyle Armstrong, Majlinda Lako
Investigative Ophthalmology & Visual Science. 2020; 61(7)

Keywords

RETINAL ORGANOIDS | MICROGLIA | IMMUNOCOMPETENT

Room temperature shipment does not affect the biological activity of pluripotent stem cell derived retinal organoids

Maria Georgiou et al.
PLoS ONE. 2020; 15(6), e0233860. 

Keywords

RETINAL ORGANOIDS | TRANSPORTATION | iPSCs

Human-induced pluripotent stem cells generate light responsive retinal organoids with variable and nutrient-dependent efficiency

Dean Hallam, Gerrit Hilgen, Birthe Dorgau, Lili Zhu, Min Yu, Sanja Bojic, Philip Hewitt, Michael Schmitt, Marianne Uteng, Stefan Kustermann, David Steel, Mike Nicholds, Robert Thomas, Achim Treumann, Andrew Porter, Evelyne Sernagor, Lyle Armstrong, Majlinda Lako
Stem Cells. 2018; 36(10):1535-1551

Keywords

RETINAL ORGANOIDS | DRUG SCREENING | iPSCs

Articles

Application of organoid technology for retinal disease modelling and drug discovery

Valeria Chichagova
Drug Target Review. June 2020

Keywords

DRUG DISCOVERY | RETINAL ORGANOIDS | DISEASE MODELLING

Posters and poster presentations

Human induced pluripotent stem cell (hiPSC)-derived retinal model containing microglial cells as a platform for toxicology studies

Valeria Chichagova, Maria Georgiou, Madeleine Carter, Birthe Dorgau, Evelyne Sernagor, Lyle Armstrong, Majlinda Lako
SOT 2020

Keywords

MICROGLIA | hiPSCs | TOXICOLOGY STUDIES

Technical sheet

iPSC-Derived Retina Organoids & Companion RPE

Technical sheet

LUNG

Articles

Lung Model Aids SARS-CoV-2 Research

Dr Mike Nicholds sat down with Technology Networks
Technology Networks. April 2020

Keywords

COVID-19 | SARS-COV-2 | HUMAN LUNG AIRWAY EPITHELIUM

Development of a physiologically relevant lung model for understanding SARS-CoV-2 infection

Professor Lyle Armstrong
Drug Target Review. November 2020.

Keywords

COVID-19 | HUMAN LUNG EPITHELIUM MODEL | SARS-COV-2

Webinars

A human iPSC-derived model of SARS-CoV-2 infection and pathogenesis in the lung

Professor Lyle Armstrong
November 2020

Keywords

SARS-COV-2 | iPSC | VIRAL INFECTION

Posters and poster presentations

Optimisation of induced pluripotent stem cell (iPSC) derived lung airway models for toxicity testing and risk assessment evaluation

Ivo Djidrovski, Katie Anderson, Lyle Armstrong
ESTIV 2018

Keywords

qPCR | LUNG ORGANOIDS | IMMUNOCYTOCHEMISTRY

Technical sheet

iPSC-Derived Upper Airway Model

Technical sheet