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Home › Models › Lung Airway Models › Human Lung Fibroblasts

Human Lung Fibroblasts

Model formats

  • Human lung fibroblasts
  • Myofibroblast transition

Cell types

  • Primary human lung fibroblasts (HLF)

Species

  • Human (Healthy donor)
  • Human (IPF donor)

Human fibroblast activation assay to assess the potential of anti - fibrotic therapeutics.

Newcells’ human primary lung fibroblasts form the basis of our fibroblast-to-myofibroblast (FMT) assay,  which allows high throughput in-vitro evaluation of anti-fibrotic therapeutics.

Following injury, the activation and transition of fibroblasts to myofibroblasts, characterized by the incorporation of α-smooth muscle actin (αSMA) into cellular stress fibers, promotes an increased synthesis and deposition of extracellular matrix (ECM) proteins, such as collagen I, to enable wound repair. Exaggerated and prolonged activation of repair mechanisms is known to be a key contributor to lung fibrosis.

Newcells’ FMT-assay replicates these changes in-vitro,  mimicking fibroblast activation and matrix deposition.

 

Available analytical readouts

High-throughput Imaging
  • Cell number
  • Proliferation rates by (cellular EdU incorporation)
  • Quantification of collagen I expression and deposition
  • Quantification of α-SMA expression
Additional readouts
  • RNA expression changes
  • Quantification of secreted cytokines/biomarkers
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Human Lung Fibroblasts stimulated with TGF-β for 72 hours and immunostained to detect collagen I, as a measure of extracellular matrix production and deposition.
Human Lung Fibroblasts stimulated with TGF-β for 72 hours and immunostained to detect collagen I, as a measure of extracellular matrix production and deposition.

Origin

Healthy and IPF donor

Assay window

72 h

Lead time

2 to 4 months

Newcells' FMT - Assay

Accelerate your lead compound selection by understanding compound efficacy against fibrosis relevant pathology.

1.

Evaluate therapeutic candidates targeting idiopathic pulmonary fibrosis (IPF) and fibrosing lung disease.

2.

Determine compound efficacy using high-throughput imaging and reliable quantification.

3.

Establish translatability between RNA expression and protein expression changes.

Introducing our Fibroblast-to-Myofibroblast Transition (FMT) Assay

Applications for human lung fibroblast model Close Open

Newcells’ FMT-Assay 

Accelerate your lead compound selection by understanding compound efficacy against fibrosis relevant pathology.

1. Evaluate therapeutic candidates targeting idiopathic pulmonary fibrosis (IPF) and fibrosing lung disease

2.  Determine compound efficacy using high-throughput imaging and reliable quantification

3.  Establish translatability between RNA expression and protein expression changes.

 

Human lung fibroblast model Close Open

Newcells have developed a high-throughput model of FMT for the study of fibroblast activation and collagen production in lung fibroblasts following stimulation with the well-established fibrotic mediator, TGF-β. Our assay set-up allows testing of small molecules and biologics for their ability to prevent fibroblast activation and ECM deposition across multiple concentrations. Through duplexed detection methods, we can simultaneously quantify cell number, αSMA and collagen I expression, maximizing experimental yields. Our FMT-assay enables further understanding of compound efficacy to advance drug-discovery programs.

 

Validated human lung fibroblasts 

  • Primary, low passage, fibroblasts isolated from adult donor lung tissue
  • Validated donors respond to TGF-β stimulation and reproducibly undergo FMT
  • Assay setup uses multiple donors to better understood patient-to-patient variability
Human lung fibroblast description Close Open
  1. Low passage normal human primary fibroblasts isolated from adult lung tissue
  2. Available from multiple donors including both healthy and IPF (idiopathic pulmonary fibrosis) donors
Human Lung Fibroblasts stimulated with TGF-β for 72 hours and immunostained to detect α-SMA, as a measure of fibroblast to myofibroblast transition.

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Fibroblasts to Myofibroblast transition
Human Lung Fibroblasts stimulated with TGF-β for 72 hours and immunostained to detect α-SMA, as a measure of fibroblast to myofibroblast transition.
Human Lung Fibroblasts stimulated with TGF-β for 72 hours and immunostained to detect collagen I, as a measure of extracellular matrix production and deposition.
Human Lung Fibroblasts stimulated with TGF-β for 72 hours and immunostained to detect Collagen I, as a measure of extracellular matrix production and deposition.

How to use our primary lung fibroblast model?

Outsource your experiments to us

Take advantage of our expertise: with our fast and reliable service, our in-house experts collaborate with you to design experiments focused on your hypothesis. Performed at our cutting-edge facilities by our team of lung experts, our fast and reliable service provides study data that presents key insights into the ability of anti-fibrotic therapeutics to reduce fibroblast activation and matrix deposition in - vivo

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Newcells Biotech's FMT Assay

Our high-throughput assays permits the screening of multiple, anti-fibrotic test articles over a range of concentrations. Our streamlines process generates experimental readouts within 2-3 months from study initiation.

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