Lung Toxicity Service

Lung Toxicity Assay

Accelerate your lead compound selection by evaluating their safety and lung toxicity profile in a relevant model of the lung small airways

Accurately investigate response to epithelial damage

Easily evaluate lung safety/toxicity in vitro

De-risk drug discovery

Newcells has developed an air-liquid interface (ALI) culture as a valuable tool for modelling the small airways of the lung in vitro and evaluating the lung toxicity of potential drug candidates

Lung toxicity and lung fibrosis occur as a result of inhalation of particles, pathogens and therapeutics but also following repeated administration of systemic and oral drugs.

Any epithelial damage or loss of epithelial barrier integrity leads to an increased susceptibility to infection, an impairment of gas exchange and triggers an immune response leading to inflammation, tissue damage and possibly lung toxicity.

Lung toxicity service: this study utilises our in vitro epithelial cell culture SAEC model to provide predictive data and enabling accurate predictions of the potential cellular toxicity of therapeutics by assessing multiple epithelial damage parameters.

Service outputs

LDH release assay
ATP activity quantification
MTT activity
TEER

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small airway lung — Small Airway Epithelial Cells immunostained to detect junctional protein ZO1, a marker for tight junctions

Multiple readouts

All major cell types

of the lung epithelia

24-well format

Air-liquid interface

Assays

LDH release
ATP assay
MTT assay
TEER

Models

Human small airway epithelial cells on air liquid interface (ALI) (24 well)

Timeline

2-4 months

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Service overview Close Open

Cultured on 3D permeable supports, our fully differentiated, polarised, human small airway epithelial cell (SAEC) model allows the addition of xenobiotics to either the apical or basolateral compartments to evaluate how they affect the epithelial barrier integrity and if they trigger cytokine release in response to cell damage.

As a standard, we measure four readouts our assays to determine drug-induced lung toxicity. As an example, Puromycin, an antibiotic with known toxic side effects in the lung, triggers a dose dependent response as measured by TEER, LDH release and ATP/MTT activity.

Puromycin induces a dose-dependent response as measured by A) TEER, B) LDH release, C) cellular ATP activity and D) formazan formation. After stimulating Newcells’ SAEC-ALI model with specified concentrations of Puromycin for 72 hours, resultant cell and epithelium damage show a dose-dependent decrease in TEER, which is associated with an increase of LDH release. Data shows a dose-dependent reduction in ATP activity and cell viability.


Models	Primary small airway epithelial cell model with an ALI (air liquid interface). The model includes all major cell types of the lung epithelium: 1. Basal cells 2. Club cells 3. Goblet cells 4. Ciliated cells
Assay format	24-well
Species	Human
Assay readout	TEER
Assay readout	Cell viability (LDH, ATP, MTT)
Biological variation	3 human healthy donors (recommended)
Technical replicates	3 technical replicates per condition (recommended)
Assay treatment window	72 hours
SKU No.	LSSAEC000H

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