Our client wished to determine the effect of three compounds on Fibroblast-to-Myofibroblast transition to determine their anti-fibrotic properties. They were looking for a supplier who could perform high content imaging (HCI) for the analysis.
The client wished to obtain detailed insights into the anti-fibrotic properties of their compounds including quantitative dose responses and how each compound affected alpha-smooth muscle actin and extracellular collagen I deposition using an FMT assay
The experimental plan involved testing the effect of three compounds on simulated primary lung fibroblasts. The fibroblasts were exposed to the compounds prior to activation by external stimuli to induce fibrosis. The results were captured and analysed by High content imaging (HCI) techniques to quantify the expression of extracellular collagen I and alpha-smooth muscle actin expressionn.
The study comprised an experimental phase, followed by data processing, analysis, and delivery of a summary report to the client.
DATASET: 3 test articles, 3 positive controls, 10 concentrations per test article, 6 technical replicates per condition.
The experiment was carried out in 384-well plates and for each data point, alpha-smooth muscle actin and collagen I deposition was quantified by immunofluorescence
The study, containing over 1000 data points, was completed in just over a month post contract agreement.
We delivered a robust dataset which contained, raw and normalised data, statistical analysis, including representative immunofluorescence images to the customer.
We consulted with our client on the interpretation of the data and a full report was drafted containing all the requested information.