A Live Cell High Content Imaging Assay for Monitoring Large Molecules Uptake into aProximateTM Proximal Tubule Epithelial Cells via Megalin and Cubulin

The proximal tubules of the kidney nephron play a key role in the reabsorption of filtered amino acids, glucose, solutes, and low molecular weight proteins, such as albumin, from the tubular filtrate. Many xenobiotics are also reabsorbed here, which if not rapidly cleared from the cells, can cause nephrotoxicity.