An in vitro Human Primary Thick Ascending Limb Model For The Assessment of Compound Nephrotoxicity

Drug-induced nephrotoxicity in the TALH has been observed with various drugs such as loop diuretics. These include electrolyte imbalances (hypokalemia, hypomagnesemia, hypocalcemia), as well as acid/base disturbances (hypochloremic alkalosis). Recently, a few Antisense Oligonucleotides (ASO) undergoing clinical research have been associated with the impairment of renal function consistent with TALH dysfunction. We have developed an in vitro primary human TALH cell model that can be used to evaluate the interaction of drug molecules such as ASOs with the TALH.