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In vitro evaluation of ocular toxicity and phenotypic rescue

The problem and data requirement

Our client wished to evaluate the retinal toxicity of a pair of lead compounds and understand how each affects the retina.

Newcells Biotech to develop 3D retinal organoids using hiPSC derived skin cells


Step 1:

Our experts worked with the customer to understand the problem

The client needed a retina model to rapidly evaluate the retinal toxicity of two molecules and understand possible differences. We proposed to determine how each compound affects cell viability as well as the cellular composition of our iPSC-derived retinal organoid model.

Step 2:

We designed a study protocol and design that efficiently provided data of value

The experimental plan involved testing compound induced toxicity using mature healthy retinal organoids differentiated for 150 days exposing the organoids for 72 hours. The readouts included retinal organoid viability assays and immunofluorescence with 6 key retinal markers to assess the organoid cells composition (ganglion cells, photoreceptors, amacrine cells, horizontal cells and Muller glia).

Step 3:

From initiation to data in less than two months

The study includes the experimental phase, data processing and analysis as well as a data summary presentation.

DATASET: 4 compounds, 1 positive control, 100 retinal organoids, 7 immunofluorescence staining per compound, ATP depletion and LDH release for each compound

The study was completed in just under 2 months.

Step 4:

We deliver a detailed report with reliable dataset

We delivered a robust dataset which contained a large set of immunofluorescence images

Outcomes for the client

Our analysis confirmed retinal toxicity of one of the compounds and we provided insights to the client as to how the compound affects different cell types.

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