Development of iPSC derived Conducting Airways and application to toxicity testing

Newcells Biotech have been awarded a grant to employ a PhD student to help develop models of the human lung from iPSCs. The student will acquire a unique multidisciplinary skill set giving them a competitive employment advantage in safety assessment sciences either in industry, governmental bodies or academia.

The in3 project is funded by the EU’s Marie Skłodowska-Curie Action – Innovative Training Network (MSCA-ITN), part of a 3-year project to develop novel ways to assess toxicity for drug development in the brain, lung, liver and kidney.

Potential outcomes from the Newcells funding:

 

  • An iPSC derived conducting airway differentiation protocol in an air-liquid interface suitable for toxicity testing
  • The model will be characterised with respect to drug and nanoparticle handling and tissue specific expression of ciliated epithelia, Clara, goblet, and basal cells. The model will be challenged with toxins and nanoparticles to investigate application to toxicity scenarios and to identify the window of opportunity for exposure
  • Investigation of donor specific effects, initially using donors with P-gp SNPs
  • To challenge the optimised system to the in3 compounds and perform mechanistic and kinetic studies
  • Deployment of reporter iPSC and testing it with a larger compound set
  • To create and optimise a pulmonary AOP in collaboration with ESR8 working on conducting airways this will involve a systematic review to identify hazards from chemical and nanomaterial inhalation exposure
You might also be interested in…
News

Development of functioning in vitro lung assay for modelling Covid-19 infection

Newcells Biotech, experts in induced pluripotent stem cell (iPSC) differentiation and assay construction, have confirmed their human lung epithelium model as an effective platform for modelling SARS-CoV-2 infection and replication.  Their work alongside the Liverpool School of Tropical Medicine (LTSM) Centre for Drugs and Diagnostics will be crucial in helping

The Biosphere
News

Newcells Biotech scales up its operations in The Biosphere

Newcells Biotech, a pioneering life-sciences spin-out that applies Human induced Pluripotent Stem Cells (hiPSCs) to develop assays for drug development, have consolidated their two laboratory sites into 500m2 of laboratory and co-located office space in The Biosphere, which forms part of the Newcastle Helix cluster.    The purpose-built space in the city centre site

COVID-19 Vaccine
Blog

An Overview of Vaccine Development & Sub-Types

Vaccination can be regarded as one of biomedicines biggest triumphs. Since Edward Jenner pioneered the smallpox vaccine in 1798, subsequent morbidity and mortality rates have declined for a multitude of diseases, such as Diphtheria, Whooping Cough and Measles.1 However, despite the success of these global vaccination