Retina - Newcells

Retinal platform

An in vitro, light responsive, retinal model for accurate predictions of in vivo outcomes you can have confidence in.

aProximateTM

Kidney platform

The most advanced near-physiological high throughput kidney proximal tubule cells (PTC) model to investigate drug transport modalities in vitro.

Lung epithelia model

Lung model

A model to investigate airway physiology, viral infection, drug safety and environmental impacts on lung airway epithelia.

Sinusoid iPSC-derived Liver model

Liver model

We are developing a model of liver sinusoid derived from human induced pluripotent stem cells (iPSC).

Retina

An in vitro, light responsive retinal model for accurate predictions of in vivo outcomes you can have confidence in. 

The retinal organoids model from Newcells recapitulates the complex structure of the retina.

Retina: a complex organ

Retina is a sensory tissue that lies at the back of the eye and is vital for visual perception. It converts light energy into electrical signals that are sent to the brain for processing. The architecture of the retina is complex and is comprised of several key cell types. Photoreceptors (rods and cones) are key light-sensing cells; other cell types include retinal ganglion, amacrine, bipolar, horizontal and Muller glial cells. Retinal pigment epithelial cells support the photoreceptors and form one of the blood-retinal barriers.

Diagram of the retina
Schematic representation of the retina

The driving force behind the development of our Retinal Platform

NEEDS for preclinical Retinal Organoids RESULTS with NEWCELLS RETINAL PLATFORM 
Recapitulation of the complex architecture with numerous cell types Functional light responsive model for mechanistic insights 
Unlimited material for use in safety and efficacy studiesSimple preclinical studies for safety and efficacy 
Lack of suitable models for human retina for disease modelling for accurate preclinical data Predictive disease modelling platform  
Ethical responsibility related to the 3R principles (Replace, Reduce and Refine) Reduction of use of animal models in line with NC3Rs and NA3RsC 

Cell types found in the differentiated Retinal Organoids

Cells found in the retinal organoids
Retinal organoids from human induced pluripotent stem cells

More information on the retinal organoids page

References for this page
Hallam, D. et al. “Human-Induced Pluripotent Stem Cells Generate Light Responsive Retinal Organoids with Variable and Nutrient-Dependent Efficiency.” Stem cells (Dayton, Ohio) vol. 36,10 (2018): 1535-1551. doi:10.1002/stem.2883

  
Chichagova, V. et al.. Cellular regeneration strategies for macular degeneration: past, present and future. Eye (Lond). 2018;32(5):946-971

 
Chichagova V. et al., . Differentiation of Retinal Organoids from Human Pluripotent Stem Cells. Curr Protoc Stem Cell Biol. 2019 Sep;50(1):e95. doi: 10.1002/cpsc.95. PMID: 31479596.


Chichagova, et al. “Human iPSC differentiation to retinal organoids in response to IGF1 and BMP4 activation is line- and method-dependent.” Stem cells (Dayton, Ohio) vol. 38,2 (2020): 195-201. doi:10.1002/stem.3116  

State-of-the-art 2D and 3D cell-based assays

RESOURCES:  

Application of organoid technology for retinal disease modelling and drug discovery; Chichagova, Drug Target Review, June 2020 
Application of organoid technology for retinal disease modelling and drug discovery (drugtargetreview.com) 

Human iPSCs generate light responsive retinal organoids with variable and nutrient dependent efficiency; Hallam et al, Stem Cells, 2018, 36(10), 1535-1551 
Human-Induced Pluripotent Stem Cells Generate Light Responsive Retinal Organoids with Variable and Nutrient-Dependent Efficiency – PubMed (nih.gov) 

Room temperature shipment does not affect the biological activity of iPSC derived retinal organoids; Georgiou et al, PLOS One, 15(6), e0233860 
Room temperature shipment does not affect the biological activity of pluripotent stem cell-derived retinal organoids (plos.org) 

Enhancing immune function of hiPSC derived retinal organoids by incorporating microglial cells; Chichagova et al, Investigtive Ophthamology and VISUAL Science, 2020, 61(7) 
Enhancing immune function of hiPSC-derived retinal organoids by incorporating microglial cells | IOVS | ARVO Journals 

Human iPSC differentiation to retinal organoids in response to IGF1 and BMP4 activation is line and method dependent; Chichagova et al, Stem Cells, 2020, 38(2), 195-201 
Human iPSC differentiation to retinal organoids in response to IGF1 and BMP4 activation is line- and method-dependent – PubMed (nih.gov) 

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